Redução relativa da motilidade twitching de Pseudomonas aeruginosa TGC-04 exposta à cafeína
DOI:
https://doi.org/10.53660/716.prw1907Palavras-chave:
bioativos naturais, biofilme, motilidade, atividade antimicrobianaResumo
A motilidade twitching deixa Pseudomonas aeruginosa mover por sobre superfícies bióticas e abióticas, permitindo sua rápida colonização em novas superfícies. Perturbações na motilidade twitching influenciam a adesão. Este trabalho teve por objetivo verificar a ação da cafeína sobre a motilidade da linhagem P. aeruginosa TGC-04 e em seguida, avaliar a adesão da linhagem exposta à 2064 µg/mL de cafeína, previamente irradiada com luz de LED de alta potência a 700 nm por 10 minutos (1000 mW/cm2). Foram realizados testes de motilidade swarming, swimming e twitching por 48h à 30ºC. O biofilme formado após incubação nas mesmas condições foi quantificado pelo método do cristal violeta. A taxa de adesão foi calculada pela diferença da absorbância do tratamento, em relação ao controle. Não houve perturbação nas motilidades swarming e swimming, contudo, a motilidade twitching foi reduzida ≈44%, refletindo na diminuição entre ≈60 e 80% da adesão das células, cujo grau de inibição foi considerado moderado, com as células moderadamente aderidas à superfície. Em complemento, foram identificadas células tipo swarm.
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