Evaluation of desmoplasia in pancreatic and colon tumors by morphometric analysis
DOI:
https://doi.org/10.53660/203.prw209Palavras-chave:
Desmoplastic reaction, Cancer, Extracellular matrix, Fibrosis, Gomori's trichromeResumo
Abstract: Objectives: This study aims to quantify collagen deposition in the desmoplasia of pancreatic and colonial tumors. Methods: Samples of colon and pancreas tumors were selected from the Hospital das Clínicas de Pernambuco and submitted to trichomy with Gomori's Trichrome. Photomicrographic analysis was performed using the integrated Panthera imaging analysis system L. Results: It was identified that 31 colon tumors and 22 pancreatic tumors presented desmoplasia with the visualization of fibrillar collagen distributed irregularly around the tumor. There was a statistically significant difference (p = 0.0031) between collagen fiber deposition in desmoplastic and non-desmoplastic pancreatic tumors, as well as for colonic tumors (p < 0.001). Conclusion: It was observed that more than half of the tumors analyzed presented desmoplasia with a difference of density of collagen fibrils statistically significant.
Downloads
Referências
Apte MV, et al. A major role for stellar cells in the microenvironment of pancreatic cancer. Gastroenterology. 144(6): 1210-1219, 2013. NIH PMID: 23622130
Bassagañas S, et al. Regulation of Glycoilation of Pancreatic Cancer Cells regulates cell adtake and invasion through Integrin Function Modulation and E-Cadherin A2b1. PLOS ONE. 9(5):e98595, 2014. PubMed PMID: 24878505
Bougort I, et al. Reciprocal interaction between Fibrillar Collagens and Collagen Binding Integrins: Implications in The Progression of Cancer and Metastasis. Borders in Oncology. 10:1488, 2020. PubMed PMID: 33014790
Brauchle E, et al. Biomechanical and biomolecular characterization of extracellular matrix structures in human colon carcinomas. Matrix Biol. 68-69, 180-193, 2018. PubMed PMID: 29605717
Carapuça EF, et al. Anti-stroma treatment, together with chemotherapy, targets multiple signaling pathways in pancreatic adenocarcinoma. J Pathol. 239(3):286-296, 2016. PubMed PMID: 27061193
Conklin MW, et al. Aligned collagen is a prognostic signature for survival in human breast carcinoma. Am J Pathol, 178(3):1221-32, 2011. PubMed PMID: 21356373
Drifka CR, et al. Highly aligned stromal collagen is a negative prognostic factor that follows pancreatic ductal ductal resection. Oncotarget, 7(46):76197-76213, 2016. PubMed PMID: 27776346
Eble JA, Niland S. The extracellular matrix in tumor progression and metastasis. Clinical & Experimental Metastasis, (3):171-198, 2019. PubMed PMID: 30972526
García-Pravia C, et al. Overexpression of COL11A1 by Cancer-Associated Fibroblasts: Clinical Relevance of a Stromamal Marker in Pancreatic Cancer. PLos ONE, 23;8. 10, 2013. PubMed PMID: 24194920
Giussani M, et al. Remodeling of the Extracellular Matrix of the Tumor: New Perspectives as a Circulating Tool in the Diagnosis and Prognosis of Solid Tumors. Cells. 8. 2:81, 2019. PubMed PMID: 30678058.
Grady WM, Markowitz SD. The molecular pathogenesis of colorectal cancer and its potential appendability to colorectal cancer screening. Dig Dis Sci, 60(3):762-72, 2014. PubMed PMID: 25492499
INSTITUTO NACIONAL DE CÂNCER – INCA. Cancer Types, 2022. [Acessed July 26, 2022]. Available in https://www.inca.gov.br/tipos-de-cancer/cancer-de-intestino .
Jang I, Beningo KA. Integrins, CAFs and Mechanical Forces in Cancer Progression. Cancers, 11(5):721, 2019. PubMed PMID: 31137693
Jayasingh C, Simiantonaki N, Kirkpatrick, CJ. Histopathological features predict metastatic potential in locally advanced colon carcinomas. BMC Cancer. 15:14, 2015. PubMed PMID: 25603809
Kalantzis I, et al. Clinical differences and correlations between right and left colon cancer. World cases J Clin. 8:1424-144, 2020. PubMed PMID: 32368535
Lobo FLR, Giglio A, Aguiar PC. Epidemiological Profile of Colorectal Cancer. Clinical oncology letters. 2020. Ahead of Print. https://doi.org/10.4322/col.2019.005.
Nebuloni M, et al. Insight into colorectal infiltration of carcinoma studying perilesional extracellular matrix. Scientific Repositions. 6:22522, 2016. PubMed PMID: 26940881
Nissen NI, Karsdal M, Willumsen N. Fibroblasts associated with Collage and Cancer in the reactive stroma and its relationship with cancer biology. J Exp Clin Cancer Res. 38(1):115, 2019. PubMed PMID: 30841909.
Rhim AD, et al. Stromative elements act to contain, rather than support, pancreatic ductal adenocarcinoma. Cancer Cell, 25(6):735-47, 2014. PubMed PMID: 24856585
Wang S, et al. Blood-based extracellular matrix biomarkers as predictors of survival in patients with metastatic pancreatic ductal adenocarcinoma receiving peg or alpha hyaluronidase. Journal of Translational Medicine. 19(1):39, 2021. PubMed PMID: 33478521
Weinberg RA, Hanahan D. Cancer Brands: The Next Generation. Cell. 144(5):646-74, 2011. PubMed PMID: 21376230.
Zenger S, et al. Clinicopathological characteristics and prognosis of histological subtypes in right-sided colon cancer. JBUON. 25(5):2154-2159, 2020. PubMed PMID: 33277830
Zippi M, et al. Desmoplasia influenced the recurrence of the disease and mortality in stage III colorectal cancer within five years of surgery and adjuvant therapy. Saudi J Gastroenterol. 23(1):39-44, 2017. PubMed PMID: 28139499
