e-QA NGS: a user-friendly tool to preprocessing data from next generation sequencing
DOI:
https://doi.org/10.53660/201.prw203Palavras-chave:
Web tool, Pre-processing, Assessment, NGS DataResumo
In the last decade, sequencing platforms have provided advances in the research developed in various omics sciences, such as genomics, transcriptomics, and metagenomics, among others. On the other hand, these platforms still face challenges, in particular the low quality of the bases at the end of the reads, which can hinder future analyses, such as the assembly process. Making it necessary to pre-process these reads where various strategies are used, such as removing low-quality bases and applying a quality filter. There are numerous software programs to help with these tasks. However, most of them execute from long and complex command lines, running on the Linux operating system, which makes it difficult for users without deep computer knowledge. This study presents e-QA NGS (http://biod.ufpa.br/eqa/), a user-friendly web tool that contributes directly to reducing the complexity of pre-processing raw reads from the sequencing process, eliminating the need for the user to install dependencies, running complex command lines, or even have a deep knowledge in the computing area.
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