Use skin as DNA in damage assessment in stranded cetaceans

Autores

  • Juliana Plácido Guimarães Centro Universitário São Judas - Campus Unimonte
  • Carlos Alexandre B. Garcia
  • Jociery E. Vergara-Parente
  • Maria Angélica Miglino
  • Durvanei A. Maria

DOI:

https://doi.org/10.53660/767.prw1905

Resumo

Environmental contamination can affect natural populations causing tissue damage such as reduced genetic variability, limiting the ability of the species live with long-term changes in the environment. Cetaceans are considered biomarkers since they are more vulnerable to exposure to environmental contaminants. This study was to analyze the phases of cell cycle in the skin of cetaceans, DNA damage and relate the results with analysis of trace elements found in these animals. We used fragments of the skin of 30 cetaceans stranded and frozen for analysis of trace elements. We observed  a large amount of iron and zinc, the latter being necessary for metalloprotease, proliferation of cutaneous cells, and deposition of collagen in wounds. Animals in an advanced state of decay showed a greater number of cells in all phases of the cell cycle, and this fact be related to a response of the organism in response to cell death.

Downloads

Não há dados estatísticos.

Referências

Ab’Saber, A.N., 2001. Litoral do Brasil. São Paulo, Metavídeo, SP Produção e Comunicação. LTDA.

Abílio, G.S., Cupelo, A.C.G., Rezende, C.E., 2004. Metal distribution in sediments of an offshore exploration area Santos basin Brazil. 4th International Symposium Environmental geochemistry In Tropical Countries R.J. Brasil, Book of Abstracts: 320-321.

Aguiar, J.E., Marins, R.V., Almeida, M.D., 2007. Comparação de metodologias de digestão de sedimentos marinhos para caracterização da geoquímica de metais-traço na plataforma continental nordeste oriental brasileira. Geochimica Brasiliensis 21(3), 304-323.

Anderson, S., Sadinski, W., Shugart, L., Brussard, P., Depledge, M., Ford, T., Hose, J., Stegeman, J., Suk, W., Wirgin, I., Wogan, G., 1994. Genetic and molecular ecotoxicology: a research framework. Environmental Health Perspectives 102 (12), 3-8.

Anzolin, D. G., Sarkis, J.E.S., Diaz, E., Soares, D.G., Serrano, I.L., Borges, J.C.G., Souto, A.S., Taniguchi, S., Montone, R.C., Bainy, A.C.D., Carvalho, P.S.M., 2012. Contaminant concentrations, biochemical and hematological biomarkers in blood of West Indian manatees Trichechus manatus from Brazil. Marine Pollution Bulletin 64, 1402-1408.

Arends, M.J., Wyllie, A.H., 1991. Apoptosis: mechanisms and role in pathology. Int Rev. Exp. Path 32, 223-254.

Augier, H., Benkoel, L., Chamlian, A., Park, W.K., Ronneau, C., 1993. Mercury, zinc and selenium bioaccumulation in tissues and organs of Mediterranean striped dolphin Stenella coeruleoalba Meyen. Toxicological result of their Interaction. Cellular and Molecular Biology 39, 621–634.

Baum, C., Meyer, W., Roessner, D., Siebers, D., Fleischer, L.G., 2001. A zymogel enhances the self-cleaning abilites of the skin of the pilot whale (Globicephala melas). Comparative biochemistry and physiology, part A: Molecular e Integrative Phisiology, Elsevier 130(4), 835-847.

Becker, P.R., Mackey, E.A., Demiralp, R., Schantz, M.M., Koster, B.J., Wise, S.A., 1997. Concentrations of chlorinated hydrocarbons and trace elements in marine mammal tissues archived in the U.S. national biomonitoring specimen bank. Chemosphere 34, 2067–98.

Bickham, J.W., Sandhu, S., Hebert, P.D.N., Chikhi, L., Athwal, R., 2000. Effects of chemical contaminants on genetic diversity in natural populations: implications for biomonitoring and ecotoxicology. Mutat. Res. 463, 33–51.

Brown, W.R., Geraci, J.R., Hicks, B.D., St. Aubin D.J., Shroeder, J.P., 1983. Epidermal cell proliferations in the bottlenose dolphin (Tursiops truncatus). Can J Zool 61, 1587-1590.

Depledge, M., 1994. Genotypic toxicity: implications for individuals and populations. Environ Health Perspect 102(12), 101-104.

Devlin, T. 2002. Manual de Bioquimica com Correlações Clínicas, Tradução da sexta edição americana, Editora Blucher.

Dieter, M. P., 1993. Identification and quantification of pollutants that have the potential to affect evolutionary processes. Environ Health Perspect 101(4), 278.

Dinsdale, D., Zhuang, J., Cohen, G.M., 1999. Redistribution of cytochrome precedes the caspase-dependent formation of ultracondensed mitochondria, with a reduced inner membrane potencial, in apoptotic monocytes. Am. J. Pathol. 155, 607-618.

Eisler, R. 2000. Chemical risk assessment handbook of Health Hazards to Humans, Plants, and Animals. Boca Raton.

Finuance, D.M., Bossy-Wetzel, E., Waterhouse, N.J., Cotter, T.G., Green, D.R. 1999. Bax-induced caspase activation and apoptosis via cytochrome c release from mitochondrial is inhibitable by Bcl-xL. J. Biol. Chem. 274, 2225-2233.

Frade, J.M., Michaelidis, T.M., 1997. Origin of eukaryotic programmed cell death: a consequence of aerobic metabolismo? Bioessays 19, 827-832.

Frankham, R., 2003. Genetics and conservation biology. Comptes rendus biologies 326, S22-9.

Fossi MC, Marsili L., 1997. The use of non-destructive biomarkers in the study of the marine mammals. Biomarkers 2, 205–16.

Fossi, M.C., Marsili, L., Neri, G., Casini, S., Bearzi, G., Politi, E., Zanardelli, M., Panigada, S. 2000. Skin biopsy of Mediterranean cetaceans for the investigation of interspecies susceptibility to xenobiotic contaminants. Marine Environmental Research 50, 517-521.

Galli, E., Feijoo, L. 2002. Citocromo p-450 y su importancia clínica revisión actualizada. Revista de Neuro-Psiquiatría 65, 187-201.

Galluzzi, L., Kepp, O., Kroemer, G. 2011. Mitochondrial dynamics: a strategy for avoiding autophagy. Curr Biol 21, R478–80.

Geraci, J.R., Lounsbury, V., 1993. Marine Mammals Ashore: a Field guide for strandings. Texas: Texas A&M Sea Grant Publications, 305p.

Green, D.R., Reed, J.C., 1998. Mitochondria and apoptosis. Science 281, 1309-1312.

Hicks, B.D., St Aubin D. J., Geraci, J.R., Brown, W. 1985. Epidermal growth in the bottlenose dolphin, Tursiops truncatus. The journal of investigative dermatology 85, 60-63.

Hoekstra, P.F., O’hara, T.M., Fisk, A.T., Borgå, K., Solomon, K.R., Muir, D.C., G. 2003.Trophic transfer of persistent organochlorine contaminants (OCs) within an Arctic marine food web from the southern Beaufort–Chukchi Seas. Environmental Pollution 124 (3), 509–522.

IBAMA. 2001. Mamíferos Aquáticos do Brasil: plano de ação, versão II. 2 ed. Brasília: Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis.

Jacobson, M.D., Burne, J.F., King, M.P., Miyashita, T. Reed, J.C., Raff, M.C., 1993. Bcl2 blocks apoptosis in cells lacking mitochondrial DNA. Nature 361, 365-369.

Kroemer, G., Zamzani, N., Susin, S.A., 1997. Mitochondrial control of apoptosis. Immunol Today 18, 44–51.

Kunito, T., Nakamura, S., Ikemoto, T., Anan, Y., Kubota, R., Tanabe, S., 2004. Concentration and subcellular distribution of trace elements in liver of small cetaceans incidentally caught along the Brazilian coast. Mar Pollut Bull 49, 574–87.

Kunito, T., Watanabe, I., Yasunaga, G., Fujise, Y., Tanabe, S., 2002. Using trace elements in skin to discriminate the populations of minke whales in southern hemisphere. Marine Environmental Research 53, 175–197.

Lacerda, L.D., Molisani, M.M., 2006. Three decades of Cd and Zn contamination in Sepetiba Bay, SE Brazil evidence from the mangrove oyster Crassostrea rhizophorae. Marine Pollution Bulletin 52, 974-977.

Maia, S.R.R., 2004. Distribuição e partição geoquímica de metais traço na costa norte de Fortaleza, CE. Tese de mestrado. Instituto de Ciências do Mar. Universidade Federal do Ceará, 105p.

Marcovecchio, J.E., 2000. Overview on land-based sources and activities affecting the marine, coastal and associated freshwater environment in the Upper Southwest Atlantic Ocean. UNEP Regional Seas Reports and Studies n° 170, UNEP/GPA coordination Office, The Hague, 78p.

Marins, R.V., Freire, G.S.S., Maia, L.P., Lima, J.P.R., Lacerda, L.D., 2002. Impacts of land-based activities on the Ceará coast, NE Brazil. In: Lacerda L.D., Kremer, H.H., Kjerfve. B., Salomons. W., Marshall-Crossland, J.I., Crossland, J.C. (eds.) South American Basins: LOICZ Global Change Assessment and Synthesis of River Catchment – Coastal Sea Interaction and Human Dimensions. LOICZ Reports e Studies n° 21, p. 92-98.

Monteiro-Filho, E.L.A., Monteiro, L.R., Reis, S.F., 2002. Skull shape and size divergence in dolphins of the genus Sotalia: a tridimensional morphometric analysis. Journal of Mammalogy 83(1), 125–134.

Moreira, I., Seixas, T.G., Kehrig, H.A., Fillmann, G., Di Benedito, A.P., Souza, C.M., Malm, O., 2009. Selenium and Mercury (total and organic) in tissues of a coastal small cetacean, Pontoporia blainvillei. Journal of Coastal Research 56, 866-870.

Nezu, R., Takagi, Y., Ito, T., Matsuda, H., Okada, A., 1999. The importance of total parenteral nutrition-associated tissue zinc distribution in wounding healing. The Japanese Journal of Surgery 29, 34–41.

Paludan-Muller, P., Agger, C.T., Dietz, R., Kinze, C.C., 1993. Mercury, cadmium, zinc, copper and selenium in harbour porpoise (Phocoena phocoena) from West Greenland. Polar Biology 13, 311–320.

Panti, C. Spinsanti, G., Marsili, L., Casini, S., Frati, F., Fossi, M.C., 2011. Ecotoxicological diagnosis of striped dolphin (Stenella coeruleoalba) from the Mediterranean basin by skin biopsy and gene expression approach. Ecotoxicology 20, 1791-1800.

Pfeiffer, C.J., Rowntree, V.J., 1996. Epidermal ultrastructure of the southern right whale calf (Eubalaena australis). Journal of Submicroscopic Cytology and Pathology 28, 277–286.

Pfeiffer C, Menon G., 2002. Cellular ultrastructural and biochemical specializations in the cetacean epidermis. In: Pfeiffer C. Molecular and cell biology of marine mammals. Melabar, Florida: Krieger Pub. Co. p. 396–411.

Saraste, A., Pulkki K., 2000. Morphologic and biochemical hallmarks of apoptosis. Cardiovascular Research 45, 528–537.

Sarkar, S., Pressey, R. L., Faith, D. P., Margules, C. R., Fuller, T., Stoms, D. M., Moffett, A., Wilson, K.A., Williams, K.J., Williams, P.H., Andelman, S., 2006. Biodiversity conservation planning tools: Present status and challenges for the future. Annual Review of Environment and Resources 31, 123–59.

Seixas, T.G., Kehrig, H.A., Costa, M., Fillmann, G., Di Beneditto, A.P.M., Secchi, E., Souza, C.M.M., Malm, O., Moreira, I., 2008. Total mercury, organic mercury and selenium in liver and kidney of a South American coastal dolphin. Enviromental Pollution 154, 98-106.

Seixas, T.G., Kehrig, H.A., Di Beneditto, Souza, C.M.M., Malm, O., Moreira, I., 2009. Trace elements in different species of cetacean from Rio de Janeiro coast. J. Braz. Chem. Soc. 20 (2), 243-251.

Stomp, A.M., 1994. Genetic information and system health: arguments for the application of chaos theory to identify boundary conditions for ecosystem management. Environ Health Perspect 102 (12), 71-74.

Tinker, S.W., 1988. Whales of the World. E.J. Brill Publishing Company, Leiden, New York, p. 310.

Yang, J., Kunito, T., Tanabe, S. Amano, M., Miyazaki, N., 2002. Trace elements in skin of Dall´s porpoises (Phocoenoides dalli) from the northern waters of Japan: an evaluation for utilization as non-lethal tracers. Marine Pollution Bulletin 45, 230-236.

Walker, C.H., Hopkin, S.P., Sibly, R.M., Peakall, D.B., 1996. Principles of Ecotoxicology, p.321.

Wilson, J.Y., Wells, R., Aguilar, A., Borrell, A., Tornero, V., Reijnders, P., Moore, M., Stegeman, J.J., 2007. Correlates of Cytochrome P450 1A1 Expression in Bottlenose Dolphin (Tursiops truncatus) Integument Biopsies. Toxicological Sciences 97(1), 111-119.

Zhuang, J., Ren, Y., Snowden, R.T., Zhu, H., Gogvadze, V., Savill, J.S., Cohen, G.M. 1998a. Dissociation of phagocyte recognition of cells undergoing apoptosis from other features of the apoptotic program. J. Biol. Chem 273, 15628-15632.

Zhuang, J., Dinsdale, D., Cohen, G.M., 1998b. Apoptosis, in human monocytic THP.1 cells, results in the release of cytochrome c from mitochondria prior to their ultradecondensation, formation of outer membrane discontinuities and reduction in inner membrane potential. Cell Death Diffrntn 5, 953-962.

Downloads

Publicado

2023-07-09

Como Citar

Guimarães, J. P., Garcia, C. A. B. ., Vergara-Parente, J. E. ., Miglino, M. A., & Maria, D. A. . (2023). Use skin as DNA in damage assessment in stranded cetaceans. Peer Review, 5(15), 215–234. https://doi.org/10.53660/767.prw1905

Edição

Seção

Articles