Methods for determining in vitro antioxidant capacity
DOI:
https://doi.org/10.53660/PRW-2216-4108Resumo
Os métodos para medir a atividade antioxidante, in vitro, são cruciais na avaliação do potencial de substâncias antioxidantes em alimentos e compostos isolados, devido a simplicidade, custo relativamente baixo e rapidez, o que permite a triagem eficiente de inúmeras amostras. Os métodos amplamente utilizados são ORAC, ABTS, DPPH, FRAP e Folin Ciocalteu. A padronização é um desafio, pois diferentes ensaios avaliam propriedades antioxidantes específicas. Os métodos in vitro, no entanto, têm limitações. Eles não replicam totalmente o complexo sistema biológico humano onde ocorrem interações dinâmicas entre antioxidantes, enzimas e radicais, mas a correlação entre resultados in vitro e in vivo nem sempre é possível. Contudo os métodos in vitro ofereçam triagem rápida e insights preliminares sobre o potencial antioxidante, e devem ser complementados por estudos in vivo. A seleção de métodos apropriados depende dos objetivos da avaliação, e a combinação de múltiplas técnicas pode fornecer informações mais abrangentes sobre as atividades antioxidantes para a promoção da saúde através dos alimentos.
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