In vivo model of colorectal cancer: impact to cigarette smoke exposition on neoplasm development
DOI:
https://doi.org/10.53660/PRW-2071-3812Palavras-chave:
Experimental carcinogenesis, Histopathological findings, Rat, Tobacco smokingResumo
Our proposal aims to investigate the impact that exposure to cigarette smoke has on the development of lesions in the colorectal mucosa of in vivo model of colorectal carcinogenesis. For that, 24 young male rats were induced to colorectal carcinogenesis and separated into two groups: exposure to cigarette smoke for 20 weeks (DMH+/Tobacco+) and control (DMH+/Tobacco-). In the group (DMH+/Tobacco+), 14.7% dysplasia and 85.29% malignant neoplasms were observed, including tubular adenocarcinoma (73%), carcinoma in situ (17%) and signet-ring cell adenocarcinoma (10%). Mononuclear inflammation and pleomorphisms were mild to moderate. In the control group, there was 7.69% dysplasia, 7.69% tubular adenoma and 84.62% malignant neoplasms, including tubular adenocarcinoma (82%), signet-ring cell adenocarcinoma (9%), carcinoma in situ (5%) and mucinous adenocarcinoma (4%). Mononuclear inflammation and pleomorphisms were moderate to severe. Thus, the experimental model showed that the lesions were predominantly malignant and with histopathological characteristics compatible with human colorectal cancer, including its possible applications for future studies.
Downloads
Referências
ALAMO, P. et al. Subcutaneous preconditioning increases invasion and metastatic dissemination in mouse colorectal cancer models. Disease Models & Mechanisms, v. 7, n. 3, p. 387-396, 2014.
BALMAIN, A.; HARRIS, C. C. Carcinogenesis in mice and human cells: parallels and paradoxes. Carcinogenesis, v. 21, n. 3, p. 371-377, 2000.
CARR, P. R. et al. Lifestyle and risk of sporadic colorectal cancer by microsatellite instability status: a systematic review and meta-analyses. Annals of Oncology, v. 29, n. 4, p. 825-834, 2018.
CABRAL, P.; BARBOSA, E. O tabaco e a doença inflamatória intestinal. Revista Portuguesa de Coloproctologia, v. 11, p. 1-6, 2014.
CHEN, H. et al. Nickel ions inhibit histone demethylase JMJD1A and DNA repair enzyme ABH2 by replacing the ferrous iron in the catalytic centers. Journal of Biological Chemestry, v. 285, n. 10, p. 7374-7383, 2010.
DREW, D. A. et al. A prospective study of the smoking risk of synchronous colorectal cancer. The American Journal of Gastroenterology, v. 112, n. 3, p. 493-501, 2017.
FLEMING, M.; RAVULA, S.; TATISHCHEV, S. F.; WANG, H. Colorectal carcinoma: Pathologic aspects. Journal of Gastrointestinal Oncology, v. 3, n. 3, p. 153-173, 2012.
GIOVANNUCCI, E. An updated review of the epidemiological evidence that cigarette smoking increases risk of colorectal cancer. Cancer Epidemiology, Biomarkers & Prevention, v. 10, n. 7, p. 725-731, 2001.
HECHT, S. S. Tobacco smoke carcinogens and lung cancer. Journal of The National Cancer Institute, v. 91, n. 14, p. 1194-1210, 2013.
HUSARI, A. et al. Juice Prevents the Formation of Lung Nodules Secondary to Chronic Cigarette Smoke Exposure in an Animal Model. Oxidative Medicine and Celullar Longevity, v. 2017, a. 6063201, 2017.
JOHNSON, R. L.; FLEET, J. C. Animal models of colorectal cancer. Cancer Metastasis Reviews, v. 32, n. 1, p. 39-61, 2013.
KOHOUTOVA, D.; PEJCHAL, J.; BURES, J. Mitotic and apoptotic activity in colorectal neoplasia. BMC Gastroenterology, v. 18, n. 65, p. 1-9, 2018.
LARANJEIRA, L. L. S. et al. Localização de lesões tumorais induzidas pela 1,2-dimetilhidrazina e seu grau de atypia no colon de ratos. Acta Cirúrgica Brasileira, v. 13, n. 3, 1998.
LIM, D. et al. Macrophage depletion protects against cigarrete smoke-induced inflammatory response in the mouse colon and lung. Frontiers in Physiology, v. 9, n. 47, p. 1-14, 2018.
LIU, E. S. et al. Cigarette smoke exposure increases ulcerative colitis-associated colonic adenoma formation in mice. Carcinogenesis, v. 24, n. 8, p. 1407-1413, 2003.
LIU, X. et al. Dose-response relationships between cigarette smoking and kidney cancer: A systematic review and meta-analysis. Critical Reviews in Oncology/Hematology, v. 142, p. 86-93, 2019.
LI, X. et al. Association of Mixed Lineage Kinase Domain-Like Protein Expression with Prognosis in Patients with Colon Cancer. Technology in Cancer Research & Treatment, v. 16, n. 4, p. 428-434, 2017.
LUGO, A. et al. Strong excess risk of pancreatic cancer for low frequency and duration of cigarette smoking: A comprehensive review and meta-analysis. European Journal of Cancer, v. 104, p. 117-126, 2018.
LUGO, A.; PEVERI, G.; GALLUS, S. Should we consider gallbladder cancer a new smoking-related cancer? A comprehensive meta-analysis focused on dose-response relationships. International Journal of Cancer, v. 146, n. 12, p. 3304-3311, 2019.
NAUSS, K. M.; LOCNISKAR, M.; PAVLINA, T.; NEWBERNE, P. M. Morphology and distribution of 1, 2-dimethylhydrazine dihydrochloride-induced colon tumors and their relationship to gut-associated lymphoid tissue in the rat. Journal of The National Cancer Institute, v. 73, n. 4, p. 915-924, 1984.
PAIVA, S. A. R. et al. Comportamento de variáveis cardíacas em animais expostos à fumaça de cigarro. Arquivo Brasileiro de Cardiologia, v. 81, n. 3, p. 221-224, 2003.
PERŠE, M.; CERAR, A. Morphological and molecular alterations in 1,2 dimethylhydrazine and azoxymethane induced colon carcinogenesis in rats. Journal of Biomedicine & Biotechnology, a. 473964, 2011.
PARK, H. S.; GOODLAD, R. A.; WRIGTH, N. A. The incidence of Aberrant crypt foci and colonic carcinoma in domethylhydrazine-treated rats varies in a site-specific manner and depends on tumor histology. Cancer Research, v. 57, n. 20, p. 4507-4510, 1997.
POHL, C.; HOMBACH, A.; KRUIS, W. Chronic inflammatory bowel disease and cancer. Hepatogastroenterology, v. 47, n. 31, p. 57-70, 2000.
SHEN, J. et al. The co-regulatory role of 5-Lipoxygenase and cycolooxygenase-2 in the carcinogenesis and their promotion by cigarette smoking in colons. Current Medicinal Chemestry, v. 23, n. 11, p. 1131-1138, 2016.
SIEGEL, R. L. et al. Global patterns and trends in colorectal cancer incidence in young adults. Gut, v. 68, n, 12, p. 2179-2185, 2019.
SUMAN, S. FORNACE, J. R., DATTA, K. Animal Models of Colorectal Cancer in Chemoprevention and Therapeutics Development. In: Ettarh R, editor. Colorectal Cancer - From Prevention to Patient Care, 1th Edit. Londres: InTechOpen: 2012. p.277-301.
SUZUKI, J. et al. Clinicopathological characteristics associated with necrosis in pulmonary metastases from colorectal cancer. Virchows Archiv: An International of Pathology, v. 474, n. 5, p. 569-575, 2019.
TRIVILIN, L. O. et al. Exposure to cigarette smoke alters AgNOR number and HIF-1alpha expression in colorectal tubular adenocarcinoma in rats. International Journal of Clinical Experimental Pathology, v. 10, n. 3, p. 3822-3829, 2017.
ULLMAN, T. A.; ITZKOWITZ, S. H. Intestinal inflammation and cancer. Gastroenterology, v. 140, n. 6, p. 1807-1816, 2011.
VERCAMMEN, D. et al. Inhibition of caspases increases the sensitivity of L929 cells to necrosis mediated by tumor necrosis factor. The Journal of Experimental Medicine, v. 187, n. 9, p. 1477-1485, 1998.
VERSCHUERE, S.; DE SMET, R.; ALLAIS L.; CUVELIER, C. The effect of smoking on intestinal inflammation: What can be learned from animal models? Journal of Crohn’s & Colitis, v. 6, n. 1, p. 1-12, 2012.
WANG, J. G.; WANG, D. F.; LY, B.J.; SI, J.M. A novel mouse model for colitis associated colon carcinogenesis induced by 1,2-dimethylhydrazine and dextran sulfate sodium. World Journal of Gastroenterology, v. 10, n. 20, p. 2958-2962, 2004.
WARD, J. M.; TREUTING, P. M. Rodent Intestinal Epithelial Carcinogenesis: Pathology and Preclinical Models. Toxicologic Pathology, v. 42, n. 1, p. 148-161, 2014.
